From Concept to Proof of Concept info@advinus.com   

In vivo metabolic profile

  • Glucose tolerance tests (PO/IP/IV)
  • Insulin tolerance test
  • Mixed meal tolerance test
  • Oral lipid tolerance test
  • VLDL secretion rates
  • Insulin/glucose clamps (hyperglycemic/euglycemic-hyperinsulinemic)
  • Substrate partitioning: assessment of glycogen synthesis and lipid synthesis post glucose load
  • Energy expenditure assessment (Indirect calorimetry)
  • Biomarker assessment to support proof of activity/mechanism (multi-analyte detection)

In vivo cardiovalscular profile

  • Rat and mouse telemetry for blood pressure (BP) and ECG measurement
  • Invasive BP measurement via carotid/femoral artery in conscious and freely moving rats
  • Non-invasive BP measurement using tail cuff method

Animal Models for Efficacy Assessment

  • Diet induced obese (DIO) mice fed with diet containing 60% or 45% Kcal from fat source,
  • High cholesterol fed hamsters model of atherosclerosis
  • Spontaneous mutations
    • db/db mice and ob/ob mice
    • Zucker fatty rats and Zucker diabetic fatty rats
    • Spontaneously hypertensive rats (SHR)
  • Chemically induced
    • STZ-induced type 1 diabetic rats
    • neonatal STZ diabetic rats
    • STZ + DIO mice
    • Mild-STZ-induced Type 1 diabetic mice
  • Surgical
    • Uninephrectomized rats/mice
    • Uninephrectomy + DOCA treated rats

Ex vivo assays

  • Isolated “Working” heart to study cardiac function and metabolism
  • Isolated perfused heart (Langendorff mode) forcardiac function
  • Receptor pharmacology using isolated tissues, e.g. aortic/fundus strips

Acute inflammation:

  • Carrageenan induced rat paw edema
  • LPS induced endotoxemia
  • Air-pouch model
  • Arachidonic acid induced ear edema

Respiratory inflammation:

  • Bleomycin induced pulmonary fibrosis in C57BL/6J mice
  • Ovalbumin (OVA) and ragweed induced allergic asthma in BALB/c mice
  • LPS induced acute lung inflammation in Wistar rats

Inflammatory bowel diseases:

  • DSS induced ulcerative colitis model in mice
  • TNBS (Trinitrobenzenesulfonic acid) induced Crohn’s disease model in rats

Joint inflammation:

  • Collagen Type-II induced arthritis (CIA) in mice
  • Adjuvant induced arthritis (AIA)
  • Collagen antibody induced arthritis (CAIA)

Skin inflammation:

  • Imiquimod-induced psoriasis in BALB/c mice

Systemic Lupus Erythematosus

  • MRL/lpr mouse model of SLE
  • NZBWF1/J mouse model of SLE

Multiple Sclerosis

  • MOG35-55-induced EAE in C57BL/6 mice

Liver Fibrosis/NASH

  • CCl4-induced rat model of liver fibrosis
  • MCD diet induced liver fibrosis in mice

Kidney fibrosis

  • Unilateral Ureteral Obstruction (UUO) model in mice
  • Folic acid induced kidney fibrosis in mice

The models are supported by strong expertise in ex vivo assays, histopathology, and PD marker studies

Hemorrhoid Model In Rat

  • Croton Oil-Induced Hemorrhoid Model In Rat

Anti- Parkinson’s: Primary & Secondary Models

  • Haloperidol induced catalepsy in rat model
  • 6-OHDA induced circling behavior in nigrostriatal lesioned rats (acute and chronic )
  • Levodopa- induced dyskinesia and rotational behavior in hemiparkinsonian rats (acute and chronic)
  • Tremorine and Oxtremorine antagonism/induced tremors/ataxia/spasticity/ salivation/lacrimation/hypothermia

Anti-Depressants:

  • Tail suspension in mice
  • Despair swim test in rats/mice
  • Apo-morphine-induced hypothermia in mice
  • Novelty Suppressed Feeding (NSF) Test in rat and mice

Cognition:

  • Object recognition test

Cerebral ischemia (MCAO)

  • Middle Cerebral Artery Occlusion rat model

Behavior and Motor Coordination

  • Rotarod Method
  • Locomotor activity in open field test
  • Morris water Maze
  • Y/T Maze Test

Safety Pharmacology Core Battery

  • Irwin’s Battery test in mice and rat

Anti-Epileptic Activity

  • Pentylenetetrazole induced Convulsions

Blood-brain barrier (BBB) integrity

  • Acute effect of LPS using Evans blue

Anti-Anxiety activity

  • Staircase test
  • Elevated Maze
  • Resident-Intruder/Isolation Aggression test

Acute Pain models

  • Acetic acid induced writhing
  • Hot plate model (central) in mice and rat
  • Post surgery acute pain
  • LPS-induced inflammatory pain
  • Mild Thermal Injury Model
  • Carrageenan/Capsaicin induced thermal/mechanical hyperalgesia
  • Tail flick/Tail immersion test or other radiant heat methods

Chronic: Neuropathic pain model

  • Spinal nerve ligation (SNL) model of neuropathic pain
  • Sciatic nerve ligation (Partial) in rat model
  • CFA induced model of inflammatory pain
  • Chemotherapy (Cisplatin)-induced peripheral neuropathic pain rat model
  • Formalin-induced pain (persistent pain model)
  • Buprenorphine-induced tolerance/withdrawal in Cispaltin-induced neuropathic pain model
  • Mono-iodoacetate induced osteoarthritis

Syngeneic animal models

  • Lung cancer model in C57BL/6J mice
  • Breast cancer model in BALB/c mice
  • CT-26 Syngeneic Colon Cancer Mouse Model

Xenograft models

  • Advinus offers heterotopic (subcutaneous) xenograft models in immuno-deficient mice using
    • Cell lines provided by the client
    • > 20 cell lines available with Advinus
    • Procured from ATCC on behalf of the client
  • Following are the immuno-deficient mice available for xenograft
    • BALB/c Nude or B6 Nude (T cell deficient
    • NOD Scid (T and B cell deficient)
    • NOG or NSG mice (T, B and NK cell deficient)

Models under Development

Chemical-induced cancer model

  • Azoxymethane+DSS-induced Colorectal cancer model in BALB/c mice

Fibrosis models

A range of animal models of Fibrosis are on offer for screening anti-fibrotic activity:

  • Lung Fibrosis:
    • Bleomycin-induced lung fibrosis
  • Liver Fibrosis:
    • Carbon tetrachloride (CCl4)-induced liver fibrosis in rats
    • Methionine choline-deficient (MCD) diet-induced mouse model of nonalcoholic steatohepatitis (NASH)
  • Kidney Fibrosis
    • Unilateral ureteral obstruction (UUO)-induced kidney fibrosis
    • Folic acid-induced kidney injury and early fibrosis
  • All the models are supported by
    • Detailed histopathology analysis
    • Gene expression analysis by qPCR
    • Immuno-histochemistry
    • Cytokine estimations
    • PK-PD correlations including tissue compound concentration

Gastro-intestinal Diseases

  • Gastric Ulcers
    • Indomethacin induced ulcer model
      • Ethanol induced ulcer model in rats
      • Pylorus Ligation Induced Ulcer Model in rats
      • Cold Restraint stress Induced Ulcer (CRU) Model in rats
  • Gastric Motility
    • Gastric emptying time (Charcoal based)

Urology

  • Rodent cystometry

Ophthalmology

  • STZ-induced diabetic retinopathy in rats; wherein:
    • Biochemical parameters, mRNA expressions for biomarkers, histopathology