Advinus Therapeutics is a drug discovery and preclinical development services focused company based in Pune and Bangalore, India. Our services include comprehensive offerings from Medicinal Chemistry, to in vitro Biology, ADMET, DMPK, in vivo Pharmacology, IP assessment, cGMP process development and optimization, GLP toxicology, long-term rodent studies and regulatory documentation.

In vitro assays are critical at all stages of drug discovery, but especially early in the drug discovery process, where they form the basis for developing Structure Activity Relationships in Medicinal Chemistry. These assays include biochemical as well as cell-based assays that are representative of more complex biological organisms or processes. Advinus offers a wide range biochemical, functional (cell-based), mechanistic and ex vivo (whole tissue-based) assays that provide exceptional value in making decisions towards compound selection for pharmacological efficacy studies in relevant animal models. Several of these assays are also amenable to high-throughput screening of compound libraries.  Our in vitro biology service offerings are shown below.

Our in vitro Biology services include:

In vitro Biology
■ Molecular and Cell Biology

• Stable cell-line generation
• Cloning and overexpression: baculoviral, mammalian
• Protein purification and characterization
• Immunological techniques
• Flow cytometry
• Gene expression

■ In vitro Assays

• Functional Assays: Cell signaling (Gs, Gi, Gq, GTPγS), substrate uptake, ion flux, glycolysis, glycogenesis, immunofluorescence-based measurements of cellular events, mechanism of action and PD marker studies
• Ligand binding assays

ADMET/DMPK
■ Permeability, efflux measurements and transporter studies

In vivo Pharmacology
■ Oncology: Xenograft studies

DISCOVERY SERVICES – CELL LINES
Advinus has an experienced team of biologists with expertise in providing services using the following cell lines: