Environmental Fate & Behaviour Studies
- TG 301 – Ready Biodegradability
- TG 302 – Inherent Biodegradability
- TG 307 – Aerobic and Anaerobic Transformation in Soil
- TG 308 – Aerobic and Anaerobic Transformation in Aquatic Sediment Systems
- TG 309 – Aerobic Mineralisation in Surface Water
Adsorption/Desorption
- TG 106 – Adsorption/Desorption Using a Batch Equilibrium Method
Hydrolysis & Photolysis
- TG 111 – Hydrolysis as a Function of pH
- TG 316 – Photo-transformation in Water
Aquatic Ecotoxicity Studies (required when PEC triggers Phase II ecotoxicity testing)
- TG 201 – Alga, Growth Inhibition Test
- TG 202 – Daphnia Acute Immobilisation
- TG 211 – Daphnia Magna Reproduction Test
- TG 203 – Fish Acute Toxicity
- TG 210 – Fish Early Life Stage Toxicity
- TG 305 – Bioaccumulation in Fish
Sediment and Soil Ecotoxicity Studies (Required for substances with soil, sediment or secondary poisoning relevance)
Sediment Organisms
- TG 218 – Sediment-Water Chironomid Toxicity (Acute)
- TG 219 – Sediment-Water Chironomid Toxicity (Chronic)
Soil Organisms
- TG 207 – Earthworm Acute Toxicity
- TG 222 – Earthworm Reproduction
- TG 216 – Soil Microorganisms: Nitrogen Transformation
- TG 217 – Soil Microorganisms: Carbon Transformation
Bioaccumulation & PBT/vPvB Assessment Studies (requires PBT screening for all active substances)
- TG 305 – Bioaccumulation in Fish
- TG 123 – Partition Coefficient (Kow) Slow-Stirring Method
- TG 107 / 117 – Partition Coefficient Estimation Methods
Sewage Treatment Plant (STP) Related Studies (For refinement of PEC and removal efficiency assessment)
- TG 303A/B – Simulation Test for STP Removal
Endocrine Disruption–Relevant Studies (Triggered for endocrine-active substances)
- TG 229 – Fish Short-Term Reproduction Assay
- TG 230 – 21-day Fish Screening Assay
- TG 231 – Amphibian Metamorphosis Assay
Specialized Aquatic Tests
- TG 236 – Fish Embryo Acute Toxicity (FET)
- TG 254 – Mason Bees Acute Contact Toxicity (rarely for human medicines, but included in OECD list)
